Figure 3 in Histopathological aspects in ripe fruits of Tahiti lime Citrus citrus x latifolia (Rutaceae) affected by phytophagous mites

Figure 3. Histochemical tests applied to Tahiti lime pericarps affected by phytophagous mites (Cross sections). A–C. Pericarps affected by Polyphagotarsonemus latus, Phyllocoptruta oleivora and Schizotetranychus hindustanicus respectively. A dark brown stain can be observed due to the accumulation of polyphenols in the OEX: exocarpal layers (white arrows, Fast Blue B); D–F. Pericarps affected by Po. latus, Ph. Oleivora and S. hindustanicus respectively. A magenta stain can be observed due to the accumulation of lignin in the exocarpal layers (white arrows, Phloroglucinol acid); G–I. Pericarps affected by Po. latus, Ph. Oleivora and S. hindustanicus respectively. Callose was not detected in the exocarpal layers (Lacmoid). Melanin deposits were observed; J–L. Pericarps affected by Po. latus, Ph. Oleivora and S. hindustanicus respectively. Primary walls stain magenta and no protein or starch granules were detected (PAS-Amidoblack); M–O. Pericarps affected by Po. latus, Ph. Oleivora and S. hindustanicus respectively. Primary walls are stained purple, lignified tissues blue-green, and polyphenols brown or black (Toluidine Blue). Schizotetranychus hindustanicus lesions are restricted to feeding zones below nests (Fig. 3O) (White arrows). Peridermis formation was observed in all cases (Fig. 3A–O). CU: cuticle; IEX: inner exocarp; ME: Melanin; OEX: outer exocarp; PP: primary walls; PR: Peridermis.