Optical trapping of micro-particles and bacterial cells in single channel and flow-focusing microfluidic devices

<p><strong>Video 1</strong>&nbsp;- The video shows the flow-focusing and trapping of&nbsp;1.84&nbsp;&mu;m bacteria-sized particles flowing at a sample flow rate of 0.1&nbsp;&mu;L/min. The horizontal sheath flow rate&nbsp;1&nbsp;&mu;L/min and the vertical sheath flow rate is 0.5&nbsp;&mu;L/min. Trapping is achieved&nbsp;using a maximum laser power of 250mW.&nbsp;</p> <p><strong>Video 2</strong>&nbsp;- The video shows the flow and fluorescence trapping of 1.84&nbsp;&mu;m bacteria-sized particles flowing at a flow rate of 0.013&nbsp;&mu;L/min.&nbsp;The channel surface is treated with pluronic F-127 to prevent cell adhesion.&nbsp;</p> <p><strong>Video 3</strong>&nbsp;- The video shows the flow and trapping of 1.84&nbsp;&mu;m bacteria-sized particles flowing at a flow rate of 1 &mu;L/min. Increased flow rate results in continuous transient trapping of the cells is achieved&nbsp;at a trapping power of 250mW.&nbsp; The microchannel surface is not treated with pluronic F-127, therefore lot of particles stick to the channel surface.&nbsp;</p> <p><strong>Video 4</strong>&nbsp;- The video shows the flow&nbsp;and trapping of 1.84&nbsp;&mu;m bacteria-sized particles flowing at a flow rate of 0.013&nbsp;&mu;L/min. Trapping is achieved&nbsp;at a laser power of 250mW. The channel surface is treated with pluronic F-127 to prevent cell adhesion.&nbsp;</p> <p><strong>Video 5</strong>&nbsp;- The video shows the flow&nbsp;and trapping of <em>E. coli</em> MG1655 flowing at a flow rate of 0.013&nbsp;&mu;L/min. Trapping is achieved&nbsp;at a laser power of 250mW. The channel surface is treated with pluronic F-127 to prevent cell adhesion.&nbsp;</p> <p><strong>Video 6</strong>&nbsp;- The video shows the flow&nbsp;and trapping of <em>S. aureus</em> 6538 flowing at a flow rate of 0.013&nbsp;&mu;L/min. Trapping is achieved&nbsp;at a laser power of 250mW. The channel surface is treated with pluronic F-127 to prevent cell adhesion.&nbsp;</p>