Data from: Experimental evolution in the cyanobacterium <i>Trichormus variabilis</i>: increases in size and morphological diversity.

<p>Data deposited in this repository was collected during the investigation entitled; Experimental evolution in the cyanobacterium <em>Trichormus&nbsp;variabilis:&nbsp;</em>increases in size and morphological diversity.</p> <p>Using experimental evolution, we selected the filamentous cyanobacteria <em>T. viariabilis&nbsp;</em>larger size by means of settling selection. The experimental design consisted of 20 replicate populations, ten propagated without selection, and ten populations were&nbsp;transferred by means of settling selection. Settling selection&nbsp;consisted of centrifugation of a 1.5 mL sample of a 72h grown population at 100 g for 30 seconds and transferring the bottom 100 &micro;L of the 1.5 mL microcentrifuge tube to fresh media. The other ten&nbsp;were propagated in batch culture transferring 100 &micro;L of a mixed population to fresh media after 72h growth.&nbsp;The selection experiment was carried out for 45 transfer cycles.&nbsp;</p> <p>Data present the morphology, size, growth, and settling rate of the populations over time, and at the end of the experiment, transfer cycle 45. The population&#39;s growth cycle&nbsp;was assessed at transfer 45 over two transfer cycles. Populations were assessed by image analysis of populations&#39; microphotographs.&nbsp;</p> <p>Morphological classification of the evolved morphologies was assessed by a double-blind volunteer panel and by objective analyses of the population&#39;s microphotographs. Canonical evolved <em>Cluster </em>and <em>Tangle </em>morphologies were compared using Principal Component Analysis.&nbsp;</p> <p>Lyticase and 2% &beta;-glucuronidase/arylsulfatase treatment of the populations enables the assessment of heritability of the evolved morphologies&nbsp;starting with single cells.</p> <p>&nbsp;</p>