Supplemental Figure S4. Nitrogen losses through feces, urine and milk as a percentage of the total N intake through feed (indicated by the red line), for each treatment group (CTRL, MetLys and MetLysHis) in each period (depletion, RP-AA, cross-back).

<p><strong>Supplemental Figure S4.</strong> Nitrogen losses through feces, urine and milk as a percentage of the total N intake through feed (indicated by the red line), for each treatment group (CTRL, MetLys and MetLysHis) in each period (depletion, RP-AA, cross-back). No significant differences nor tendencies were determined between treatment groups within every period by Tukey&rsquo;s pairwise comparison test (<em>P</em> &gt; 0.10). During the whole experiment all treatment groups received a low protein diet (CTRL). The cows in the MetLys group received rumen-protected (RP) Met (Excential Rumenpass MET, Orffa Additives) and RP-Lys (AjiPro-L, Ajinomoto H&amp;N) during the RP-AA period. The cows in the MetLysHis group received RP-Met, RP-Lys and RP-His (experimental RP-His product, Ajinomoto Co.) during the RP-AA period.</p>