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3DED/MicroED datasets of hemozoin recorded by a SINGLA detector

<p>32 diffraction&nbsp;datasets were collected using a Thermo Scientific Glacios operated at 200 kV with a stage temperature of ~80 K. The gun lens was reduced to the minimum (8), and spot size (11) with a C2 aperture of 50 &micro;m&nbsp;was used. The beam was brought to parallel corresponding to a beam diameter of approximately 1.7 &micro;m. The diffraction images were recorded by a DECTRIS SINGLA detector in the h5 format. NeXus format header files&nbsp;were subsequently generated using Nexgen (https://github.com/dials/nexgen).&nbsp;The diffraction patterns were collected at a nominal camera length of 677 mm according to the microscope control software (estimated to be&nbsp;to 809 mm at the&nbsp;detector). The sample was rotated through 90&deg; over 18s with the camera collecting data at 50 Hz, producing images representing 0.1&deg;. Subsequently, the oscillation angle was refined to 0.11&deg;. The dose rate under these conditions was 0.01 e- &Aring;-2 s-1.</p> <p>A data processing script is provided to index, integrate and scale 64&nbsp;lattices across the datasets using DIALS 3.13</p>

ShareScore

44/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
8
Harmonization
4
Access
20
Reuse readiness
8
Engagement
4

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