2D LSFM timelapse of cardiomyocyte calcium dynamics

<p>Uploaded zip-folder contains the following files:<br> 1. A&nbsp;representative&nbsp;raw dataset of a 2D LSFM ventricular cardiomyocyte undergoing stimulated calcium transients and&nbsp;calcium sparks (frame_0000.tif -frame_17999.tif)<br> 2. The recorded pacing signal time trace (waveform_test.xslx)<br> 3. Image&nbsp;corresponding to the time-averaged background (AVG_19_35_39_LowNA rolling shutter.tif)<br> 4. Pre-processed nuclear mask matrix (NuclearMask.mat), CMO-channel average (CMO_Average.mat), and CMO channel maximum intensity projection (CMO_MIP).&nbsp;<br> 5. Split and co-registered data for each spectral channel (CMO_frame_00001.tif-CMO_frame_18000.tif,&nbsp;FLUO4_frame_00001.tif -FLUO4_frame_18000.tif).<br> <br> Compressed file size: 14.9 GB<br> Uncompressed file size: 42.8 GB.&nbsp;<br> <br> Related to the following manuscript:&nbsp;<br> Liuba Dvinskikh, Hugh Sparks, Ken MacLeod and Chris Dunsby &quot; <em>High-speed 2D light-sheet fluorescence microscopy enables quantification of spatially varying calcium dynamics in ventricular cardiomyocytes</em>&quot; (2023), <em>In review</em> with Frontiers in Physiology, Cardiac Electrophysiology.&nbsp;</p>