B-other ALL classification by Targeted RNA-sequencing

<p>We present a comprehensive genetic study of 144 pediatric B-other Acute Lymphoblastic Leukemia&nbsp;cases diagnosed and treated at&nbsp;Boldrini Children&#39;s Hospital (Brazil). We performed a targeted RNA-sequencing to evaluated the benefits of introducing genomic technologies into routine diagnostics.&nbsp;Targeted RNA-sequencing further classified 66.7%&nbsp;B-other cases. All &#39;classical&#39; and novel ALL subgroups, except for iAMP21, hyper- and hypodiploid cases were identified. In addition, clinically important genetic alterations as druggable lesions and prognostic factors were&nbsp;found. Here, we uploaded files that contain:&nbsp;</p> <p>1. Gene expression data from the entire cohort studied, n=184 (Log2+1 TPM normalized gene expression data). Tab-delimited file (.csv) that contains a&nbsp;row for gene, a column&nbsp;for each sample, and expression values for each gene in each sample.&nbsp;&nbsp;Annotation labels are in the first three rows and in the first column.</p> <p>2. A subset of 23 BAM (Binary Alignment/Map) files for representative ALL cases, including BCR-ABL1, ETV6-RUNX1, TCF3-PBX1, KMT2A-r, High-Hyperdiploidy; DUX4-r, iAMP21,&nbsp;PAX5-driven,&nbsp;ABL-class fusion,&nbsp;JAK2-fusion, EPOR-fusion,&nbsp;CRLF2-high,&nbsp;ZNF384-r,&nbsp;MEF2D-r,&nbsp;NUTM1-r, B-&#39;rest&#39;, IKZF1del, and ERGdel.</p> <p>3- Genetic&nbsp;information&nbsp;of the 23 BAM files provided (tab-delimited file, .txt).&nbsp;</p>