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Manual time-lapse at 50°C of DNA-droplets after addition of amphiphile

<p>Time-lapse microscopy data of DNA-nanomotifs (color channel 1) and amphiphilic nanomotifs (color channel 2), samples were incubated at 50&deg;C in a thermocycler, time-step between measurements is 10 min. Imaging was performed at an ambient temperature of 40&deg;C within the microscope enclosure. The upload contains microscope software data (NIS-elements) and the scripts used for segmentation of droplets and further analysis.</p> <p>Additional image information</p> <p>Objective: Apo LWD 40x WI &lambda;S DIC N2.</p> <p>Width:&nbsp; 232.375 microns (1430)<br> Height:&nbsp; 168.35 microns (1036)<br> Depth:&nbsp; 12.6 microns (21)<br> Size:&nbsp; 119MB<br> Resolution:&nbsp; 6.1538 pixels per micron<br> Voxel size: 0.1625x0.1625x0.6 micron^3</p>

ShareScore

24/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
4
Harmonization
4
Access
16
Reuse readiness
0
Engagement
0

Dataset details

Repository
zenodo
Published
January 25, 2023
License
cc-by-4.0
DOI
10.5281/zenodo.7572229

Scored with release 0.9.0

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