MicroED datasets of Biotin captured on a K2 detector in counting mode

<p>Continuous rotation data sets in MRC format were collected on a JEOL CryoARM300 (300 kv) equipped with a K2 summit direct electron detector. Datasets were collected from 7 different crystals. Each dataset was collected with an approximate flux of 0.009 e-/ &Aring;^2/s-1 giving a final total exposure of 2.5 e-/&Aring;^2 for a 70-degree wedge of data. All datasets were processed in DIALS and example bash scripts are provided in the SCRIPTS folder.&nbsp;Experimental parameters are as follows:</p> <p>&nbsp;</p> <p>Accelerating voltage: 300 kV</p> <p>Wavelength: 0.019687</p> <p>Cameralength: 410 mm</p> <p>Exposure time: 3 s</p> <p>Rotation speed: 0.25 degrees s-1</p> <p>Rotation: Variable - direction is noted in the files&nbsp;</p> <p>Detector area: 1919 x 1855 (Bin 2)</p> <p>Pixel size: 0.01 mm</p> <p>Spot size: 7&nbsp;</p> <p>Angle: 5</p> <p>C2 aperture: 40 &micro;m</p> <p>SA aperture: 20 &micro;m (1 &micro;m at the sample)</p>