Genome report: Genome sequence of 1S1, a transformable and highly regenerable diploid potato for use as a model for gene editing and genetic engineering

<p>Generation of a genomic resource for a readily transformable diploid potato would provide a resource for high throughput functional analysis in potato. The heterozygous <em>Solanum tuberosum</em> Group Phureja clone 1S1 has a high regeneration rate, self-fertility, desirable tuber traits and is amenable to <em>Agrobacterium</em>-mediated transformation. To create a contiguous genome assembly, a homozygous doubled monoploid of 1S1 (DM1S1) was sequenced using 44 Gbp of long reads generated from Oxford Nanopore Technologies (ONT), yielding a 736 Mb assembly that encoded 31,145 protein-coding genes. The final assembly for DM1S1 represents a nearly complete genic space, shown by the presence of 99.6% (C:99.5%[S:97.8%, D:1.7%],F:0.1%,M:0.4%,n:1614) of the Benchmarking Universal Single Copy Orthologs. Variant analysis with Illumina reads from 1S1 was used to deduce its alternate haplotype using the variant calling tools Strelka2 (v2.9.10), GATK's Haplotypecaller (v4.1.4.1), and Freebayes (v1.3.2). These variants were used to create consensus fasta sequences with the DM1S1 assembly using bcftools (v1.9.64).</p>