CRISPR/Cas9 mediated knockout of ABCC1 confers resistance to Bacillus thuringiensis toxin Cry2Ab in Helicoverpa armigera

<p>This data contains the Bt and insecticide bioassay results of ABCC1 knockout and wild-type strain of <em>Helicoverpa armigera</em>.&nbsp;Bioassay results revealed that the HaABCC1-KO strain exhibited &gt;60-fold resistance to Cry2Ab toxin compared with the susceptible XJ strain, while no resistance has been recorded for Cry1Ab, Cry1Ac and Vip3A. Furthermore, the inheritance of <em>H. armigera</em> resistance to Cry2Ab was autosomal and completely recessive, significantly associated with the 4-nt deletion mutation of ABCC1 in the HaABCC1-KO strain. The relative growth of HaABCC1-KO and XJ strain also confirmed the role of <em>HaABCC1 </em>on the toxicity of Cry2Ab.</p>