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MinION plasmid deep long read sequencing for sequence verification

<p>We sequenced four plasmid constructs, each with a whole MinION flowcell, for use in developing and testing a sequence verification procedure. The resulting pipeline can sequence verify plasmid constructs, generating a consensus sequence with associated confidence of each base, adhering to strict acceptance criteria . The data contained herein are a subset of the complete data; 30 fast5 files for each plasmid, to be used as example data.</p> <p>&nbsp;</p> <p>datHL_001519: BCRxV.TF.1<br> datHL_001521: BCRxV.VSVG.1<br> datHL_001617: BCRxV.GagPolRev.1<br> datHL_001620: BCRxV.VSVG.1_mutant</p>

ShareScore

36/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
8
Harmonization
4
Access
16
Reuse readiness
8
Engagement
0

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