Trichoderma-Arabidopsis interaction by Ambient Ionization Mass Spectrometry

<p>Interaction between the plant <em>Arabidopsis thaliana</em>&nbsp;and the fungus <em>Trichoderma atroviride</em>.</p> <p>Three sample groups (<em>Arabidopsis thaliana</em>, <em>Trichoderma atroviride</em> and <em>Trichoderma-Arabidopsis</em>&nbsp;interaction) with 3 biological replicates were monitored for 10 days, resulting in 40 points. This experiment was focused on monitoring the kinetics of the volatile organic compound (VOC) 6-Pentyl-2H-Pyran-2-One&nbsp;(6-PP), a molecule produced by <em>Trichoderma</em>&nbsp;that helps to establish interaction with plants. &nbsp;More details and results are available in the publication written by Torres-Ortega et al. (DOI: <a href="https://doi.org/10.3390/metabo12121231">10.3390/metabo12121231</a>).&nbsp;</p> <p>Data were collected with&nbsp;custom 3D-printed Low-Temperature Plasma (DOI: <a href="https://doi.org/10.1021/acs.analchem.6b01019">10.1021/acs.analchem.6b01019</a>) ambient ionization source, coupled to an LCQ-fleet ion trap (Thermo Scientific, USA). Mass spectra were taken in full spectra at 50-500 <em>m/z</em> range, on profile mode. Each MS scan consists of 10 micros cans. Data was saved under the RAW property format of Thermo. To convert to mzML open format, Proteowizard was used.</p>