Inner filter effect correction for fluorescence measurements in microplates (AddAbs) - experimental data

<p>Experimental data for the paper entitled <em>Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber</em> (<a href="https://doi.org/10.1021/acs.analchem.3c01295">https://doi.org/10.1021/acs.analchem.3c01295</a>).</p> <p>Separate worksheets are provided for the following:</p> <p>1. Fluorescence measurements - raw values in triplicate; 2 worksheets for transparent (T) and nontransparent (NT) microplates, each with 15 titrations: L₁-L₁₂ and H₁-H₃,</p> <p>2. Absorbance measurements - raw values in triplicate (<em>&lambda;</em>_ex = 345 nm, <em>&lambda;</em>_em = 390 nm); 1 worksheet for T microplates only, with 4 titrations: L₁-L₄,</p> <p>3. Fluorescence measurements - averaged, baseline-corrected averaged and normalized baseline-corrected averaged triplicates; 2 worksheets for T and NT microplates, each with 15 titrations: L₁-L₁₂ and H₁-H₃,</p> <p>4. ZINFE/NINFE-corrected fluorescence in T microplates; 12 worksheets for 12 titrations: L₁-L₉ and H₁-H₃,</p> <p>5. ZINFE/NINFE-corrected fluorescence in NT microplates; 12 worksheets for 12 titrations: L₁-L₉ and H₁-H₃.</p> <p>The results of the ZINFE and NINFE correction methods obtained using the online calculator service written in Javascript: https://ninfe.science (version 09.5.2022.)</p> <p>For additional details please visit: https://glymech.pharma.hr//GlyMech.html.</p>