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Quantification data for article entitled "ATG9 vesicles comprise the seed membrane of mammalian autophagosomes"

<p>Membrane fractionation, immunoprecipitation, and&nbsp;<em>in vitro</em>&nbsp;experiment densitometric quantification datasheets for quantified data presented in the research article entitled&nbsp;&quot;ATG9 vesicles comprise the seed membrane of mammalian autophagosomes&quot;. Membrane fractions (Figures 3, 4, S4, and S5)&nbsp;were&nbsp;from WT cells (starvation + bafiomycin A1&nbsp;and basal conditions), ATG2 DKO cells, FIP200 KO cells, ATG9A KO cells, ATG3 KO cells, and ATG4 QKO cells (all KO cells under basal conditions). Immunoprecipitations (Figures 6 and S5)&nbsp;were&nbsp;from WT cells (starvation + bafiomycin A1) and ATG2 DKO cells (basal). Guanidine hydrochloride (GdnHCl) sequential immunoprecipitations (Figure 6) were&nbsp;from WT cells (starvation + bafiomycin A1).&nbsp;<em>In vitro</em>&nbsp;experiments (Figure S5) were&nbsp;using recombinant proteins on&nbsp;reconstituted liposomes.</p>

ShareScore

32/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
4
Harmonization
4
Access
16
Reuse readiness
8
Engagement
0