Modifying the expression of cysteine protease gene PCP affects pollen develop-ment, germination and plant drought tolerance in maize

<p>Figure S1: The sequencing results of pollen grains of T2 mutants. Figure S2: The relative expression levels of <em>PCP</em> were analyzed via RT-qPCR in <em>pcp</em> mutant lines. Data represent means &plusmn; SD of three replicates. Significant differences were indicated with different letters (<em>P</em> &lt; 0.05, one-way ANOVA). Figure S3: Pictures of pollen germination and growth in <em>vitro</em> between wild type and transgenic lines after 4 h and 6 h incubation. Scale bar: 100 &mu;m; Figure S4: Phenotypic observation of wild-type and transgenic maize at flowering stage. Scale bar: 10 cm. Figure S5. DAB staining detection of ROS in WT, <em>KO</em> and <em>OE</em> plants under drought stress. Table S1: PCR primers used in this study.</p>