Figure 4 in Diversity and evolution of sexually dimorphic mental and lateral glands in Cophomantini treefrogs (Anura: Hylidae: Hylinae)

Figure 4. Light micrographs of cross-section of lateral skin regions of males of some species of Cophomantini: A, Hypsiboas pulchellus. B, Hypsiboas albomarginatus. C, Bokermannohyla pseudopseudis. D, Hypsiboas semilineatus. E, Hypsiboas heilprini. F, Aplastodiscus perviridis. G, Hypsiboas faber. H, Hypsiboas albopunctatus. I, J, K, L, Hypsiboas pombali. A, B, lateral glands are absent. Note the occurrence of only ordinary glands within the stratum spongiosum. C, D, lateral glands are characterized by the occurrence of both dimorphic skin glands, specialized mucous glands (SMGs) and specialized serous glands (SSGs), scattered in the integument. Notice differences in the size and staining properties of ordinary mucous glands (OMGs). E, F, glands are highly packed and their secretory portion disposed in a single layer. E, SMGs of the tubuloalveolar morphology. F, SMGs of the alveolar type and also SSGs. G, H, the secretory portions of glands are disposed in two layers. Notice the difference in size of the SMGs. I–L, differences in structural and histochemical properties of SMGs, ordinary serous gland (OSGs) and SSGs. Histological staining: A, B, H, Masson's trichrome; I, Masson–Goldner's trichrome; C, D, E, F, J, Alcian blue-periodic acid Schiff; G, L, Coomassie Blue R250; K, Sudan black B. sc, stratum compactum; SMG; ss, stratum spongiosum; SSG, specialized serous gland. Scale bars = 100 μm.