Microscopy-based assessment of RNA polymerase II clusters during sperm precursor formation in fruit fly testes

<p>This repository contains the data and MatLab analysis scripts of the analysis of RNA polymerase II clusters over the course of differentiation into sperm precursor cells in fruit fly testes. Fruit flies were raised in Sylvia Erhardt&#39;s laboratory, samples prepared and microscopy images recorded by Agnieszka Pancholi, and images analysed by Lennart Hilbert.</p> <p>To analyse date data, the raw image data are first extracted into MatLab-native files using the&nbsp;<a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/MultiPosition_extraction_nd2.m">MultiPosition_extraction_nd2.m</a>&nbsp;script. The actual analysis is then carried out using the&nbsp;<a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ClusterAnalysis.m">ClusterAnalysis.m</a>&nbsp;script. Example microscopy images were produced using the&nbsp;<a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ExampleImages.m">ExampleImages.m</a>&nbsp;script. The extracted data can be reviewed using the&nbsp;<a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ReviewExtractedStacks.m">ReviewExtractedStacks.m</a>&nbsp;script.</p>