Microscopy-based assessment of H3K27-acetylation at RNA polymerase II clusters during early stages of zebrafish embryo development

<p>This repository contains the data and MatLab analysis scripts of the analysis of histone 3 lysine 27 acetylation (H3K27ac) at RNA polymerase II clusters over the course of zygotic genome activation and gastrulation of zebrafish embryos. Samples were prepared and images were recorded by Marcel Sobucki&nbsp;in Lennart Hilbert&#39;s laboratory, images were analyzed by Lennart Hilbert.</p> <p>To analyse date data, the raw image data are first extracted into MatLab-native files using the&nbsp;<a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/MultiPosition_extraction_nd2.m">MultiPosition_extraction_nd2.m</a>&nbsp;script. The actual analysis is then carried out using the&nbsp;<a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ClusterAnalysis.m">ClusterAnalysis.m</a>&nbsp;script. Example microscopy images were produced using the&nbsp;<a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ExampleImages.m">ExampleImages.m</a>&nbsp;script. The extracted data can be reviewed using the&nbsp;<a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ReviewExtractedStacks.m">ReviewExtractedStacks.m</a>&nbsp;script.</p> <p>Additional data needed to be stored in an extra repository due to size limitations:&nbsp;<a href="https://doi.org/10.5281/zenodo.8028508">https://doi.org/10.5281/zenodo.8028508</a></p>