Ionome analysis of Salmonella mutants by Inductively coupled plasma mass spectrometry (ICP-MS)

<p>In many Gram-negative bacteria, the stress sigma factor of RNA polymerase, σS/RpoS, remodels global gene expression to reshape the physiology of quiescent cells and ensure their survival under non-optimal growth conditions. In the foodborne pathogen <i>Salmonella enterica</i> serovar Typhimurium, σS is also required for biofilm formation and virulence.</p><p>We have previously shown that a Δ<i>rpoS</i> mutation affects the <i>Salmonella</i> ionome. Indeed, inductively coupled plasma mass spectrometry analyses have unraveled a significant effect of the Δ<i>rpoS </i>mutation on the cellular concentration of manganese, magnesium, cobalt and potassium, suggesting that σS controls fluxes of ions that might be important for the fitness of quiescent cells (Metaane et al. 2022, PLoS ONE 17(3): e0265511).</p><p>Study: These findings prompted us to evaluate the impact on the<i> Salmonella</i> ionome of deletions of genes encoding&nbsp; the <i>Salmonella</i> Mn2+ transporters (<i>sitABCD</i> and <i>mntH</i>), the Co2+ transporter (<i>cbiMNQO</i> operon) and small proteins of unkown function (<i>yqaE</i> and <i>yqjDEK</i>) that accumulate in quiescent <i>Salmonella</i> under the tight control of σS (Levi-Meyrueis et al. PloS one. 2014; 9(5):e96918, Lago et al. Scientific reports. 2017; 7(1):2127 and Metaane et al. 2022, PLoS ONE 17(3): e0265511).</p><p>Material and Methods: Cell-associated contents of several elements were measured by inductively coupled plasma mass spectrometry (ICP-MS) as previously described in Metaane <i>et al </i>2022 PLoS ONE 17(3): e0265511.Dried cell pellets were prepared by V. Monteil and F. Norel (Institut Pasteur, Université de Paris, CNRS UMR3528, Biochimie des Interactions Macromoléculaires, F-75015, Paris, France). Cell-associated contents of several elements were measured by S. Ayrault and L. Bordier (ICP-MS platform, Laboratoire des Sciences du Climat et de l'Environnement, LSCE/IPSL, CEA-CNRSUVSQ,Université Paris-Saclay, 91191, Gif-sur-Yvette, France)</p><p><strong>This work was supported by the French National Research Agency (ANR-19-CE44-0005-01, PERIOMET project).</strong></p><p><strong>Linked studies:</strong></p><ul><li>NOREL Francoise, MONTEIL Veronique, DOUCHE Thibaut, &amp; MATONDO Mariette. (2023). Global effects of deletions of the sitABCD, mntH, cbiMNQO and corA genes, encoding transporters for manganese, cobalt and magnesium on protein abundance in Salmonella enterica serovar Typhimurium grown to stationary phase in LB. [Data set]. Zenodo. https://doi.org/10.5281/zenodo.8279780</li><li>Metaane S, Monteil V, Douché T, Giai Gianetto Q, Matondo M, Maufrais C, Norel F. Loss of CorA, the primary magnesium transporter of <i>Salmonella, </i>is alleviated by MgtA and PhoP-dependent compensatory mechanisms. PloS one. 2023;18(9):e0291736.</li></ul>