Fluorescence correlation spectroscopy TCSPC data with and without peak artifacts - AlexaFluor 488 applied experiment

<p>This is a dataset of Fluorescence Correlation Spectroscopy (FCS) Time-Correlated Single Photon Counting (TCSPC) data&nbsp;with and without peak artifacts. The provenance of the data is recorded in <a href="https://github.com/aseltmann/fluotracify/blob/data/data/exp-220120-correlate-ptu/LabBook-exp-220120-correlate-ptu.org">this file</a>&nbsp;(see a rendered version <a href="https://aseltmann.github.io/fluotracify/data/LabBook-all.html#sec-2-5">here</a>). This parent project (<a href="https://github.com/aseltmann/fluotracify">https://github.com/aseltmann/fluotracify</a>]) also contains examples of how to use these models&nbsp;and related Python code.</p> <p>The following connected paper is currently under review and should be cited together with these model versions: <em>Seltmann, A.; Carravilla, P.; Reglinski, K.; Eggeling, E.; Waithe, D. Neural Network Informed Photon Filtering Reduces Artifacts in Fluorescence Correlation Spectroscopy Data. 2023 (currently under review)</em></p> <p><strong>Note on the file formats and notation:</strong></p> <p>&quot;Primary data&quot; refers to the <code>.ptu</code> files, so the actual TCSPC data. &quot;Secondary data&quot; refers to the <code>.pqres</code> files, which are derived files by the proprietary PicoQuant software.</p> <p><strong>Note on sample preparation (from the Supplementary Note of the paper above):</strong></p> <p>The peak artifact measurements were produced by mixing 20 nM of the small dye Alexa Fluor 488 (Thermo Fisher Scientific) with 10 &mu;M of slow, large (100 nm) unilamellar vesicles (LUVs) labelled with DiO in the membrane (0.1 % mol lipid:dye). The corresponding control measurements were 20 nM Alexa Fluor 488 in solution. We prepared the LUVs by the extrusion method: 1&minus;palmitoyl&minus;2&minus;oleoyl&minus;glycero&minus;3&minus;phosphocholine (POPC, Avanti Polar Lipids) was vacuum dried on a glass vial for 1 h and resuspended on phosphate buffered saline to form lipid vesicles. The vesicles were extruded 30 times through 100 nm pore size polycarbonate membranes using a Mini-Extruder (Avanti Polar Lipids). We prepared the samples on #1.5 coverslips mounted on Attofluor Cell Chambers (Thermo Fisher Scientific). We acquired the data on a MicroTime 200 microscope (PicoQuant) equipped with an Olympus UPlanSApo 60&times; 1.2NA water immersion objective lens and a HydraHarp 400 TCSPC&nbsp;module (PicoQuant). Excitation was achieved with a 488 nm pulsed laser (PicoQuant) with a power of 5 mW measured at the sample plane. We used a 20 nM solution of Alexa Fluor for calibrating the correction collar. One TCSPC measurement had a length of 10 s for AF488 experiments.</p>