Sequences of target genes and probes for construction of exon-targeted libraries for Prunus spp.

<p>(Files)</p> <p>&quot;baits-selection-15171.fas&quot;: Sequences of 15,171 baits used for hybridization reaction.</p> <p>&quot;Targets_mume.15171.xlsx&quot;: Sequences of target genes used for design baits.</p> <p>&nbsp;</p> <p>(Protocol for bait design)</p> <p>To selectively retrieve libraries with exons, a myBaits Custom design kit was used to design 1&ndash;20-K probes (Arbor Biosciences, Ann Arbor, MI, USA), which uses biotinylated RNA probes to concentrate fragments carrying sequences of interest (Gnirke et al., 2009), based on the published genomic and coding sequences of <em>P. mume</em> (Zhang et al., 2012). We subjected 29,621 non-redundant coding loci to search for single hits with BLAST+ (MEGABLAST with -p 70) against the <em>P. mume</em> genome, for the subsequent bait design. A 120-mer bait with 25&ndash;55 GC% per locus was randomly designed for each locus, and finally we obtained a bait set targeting 15,171 coding loci.</p>