Disturbed trophoblast transition links early fetal to maternal syndrome progression in pre-eclampsia

<p>Pre-eclampsia (PE) is a syndrome that affects multiple organ systems and is the most&nbsp;severe hypertensive disorder in pregnancy. It frequently leads to preterm delivery,&nbsp;maternal and fetal morbidity and mortality, and life-long complications (1). We currently&nbsp;lack efficient screening tools (2,3) and early therapies (4,5) to address PE. To study the initial&nbsp;stages of early onset PE and identify candidate markers and pathways, we performed&nbsp;spatio-temporal multi-omics profiling of human PE placentae and healthy controls and&nbsp;validated targets in early gestation in a longitudinal clinical cohort. We used a single-nuclei RNA-seq approach, spatial proteo-, and transcriptomics, and&nbsp;mechanistic in vitro signaling analyses.</p> <p>This repository contains the single nuclei processed data&nbsp;for decidua and placenta, and&nbsp;Visium (10x spatial transcriptomics) data used in the study. It contains the anndata objects (H5AD) for snRNA-seq with required metadata and NMF-based spot deconvolution data for visium (RDS). The raw Cellranger feature-barcode matrices (H5 files) per donor sample are also included.&nbsp;</p> <p>References</p> <p>1 Williams, D. Long-term complications of preeclampsia. Semin Nephrol 31, 111-122,<br> doi:10.1016/j.semnephrol.2010.10.010 (2011).</p> <p><br> 2 Brown, M. A. et al. Hypertensive Disorders of Pregnancy: ISSHP Classification,<br> Diagnosis, and Management Recommendations for International Practice.<br> Hypertension 72, 24-43, doi:10.1161/HYPERTENSIONAHA.117.10803 (2018).</p> <p><br> 3 Zeisler, H. et al. Predictive Value of the sFlt-1:PlGF Ratio in Women with Suspected<br> Preeclampsia. N Engl J Med 374, 13-22, doi:10.1056/NEJMoa1414838 (2016).</p> <p><br> 4 Hauth, J. C. et al. Pregnancy outcomes in healthy nulliparas who developed<br> hypertension. Calcium for Preeclampsia Prevention Study Group. Obstet Gynecol 95,<br> 24-28, doi:10.1016/s0029-7844(99)00462-7 (2000).</p> <p><br> 5 Phipps, E. A., Thadhani, R., Benzing, T. &amp; Karumanchi, S. A. Pre-eclampsia:<br> pathogenesis, novel diagnostics and therapies. Nat Rev Nephrol 15, 275-289,<br> doi:10.1038/s41581-019-0119-6 (2019).</p>