Peterson-Lab_rat_OPCs_to_iNs_over_70_hours

<p>Adult rat cortex-derived oligodendrocyte progenitor cells (OPCs) were infected with retrovirus (RV) expressing Ngn2-eGFP and imaged continuously from 4-6 days post-infection (DPI) using an environmental chamber-equipped Leica SP8 resonance confocal microscope with illumination by a 488 nm laser and image collection for emission by eGFP and DIC optics.&nbsp; The video has been annotated to show a period of approximately two hours each on days 4, 5, and 6 DPI.&nbsp; The video concludes with images of fixed cultures immunostained for antibodies to the mature neuronal marker, NeuN, conjugated to a Cy3 fluorophore.&nbsp;&nbsp;</p>