Fig. 3 in An electrophysiological characterization of naturally occurring tobacco alkaloids and their action on human α4β2 and α7 nicotinic acetylcholine receptors

Fig. 3. Sequential applications of brief ACh pulses are plotted as a function of time for three ACh concentrations. (a) EC20 = 0.4 μM (circles), EC50 = 1.2 μM (squares), EC90 = 11.1 μM (triangles) for human α4β2 receptors. (b) EC20 = 33.3 μM (circles), EC60 = 300 μM (squares), EC90 = 900 μM (triangles) for human α7 receptors. Experiments were performed by using the whole-cell mode of the patch-clamp technique at a holding potential of −70 mV in CHO cells stably expressing the human α4β2 and α7 nAChRs. EC, effective concentration. Data are presented as mean ± SD.