Fig. 2 in An electrophysiological characterization of naturally occurring tobacco alkaloids and their action on human α4β2 and α7 nicotinic acetylcholine receptors

Fig. 2. Representative traces of currents elicited by using the agonist mode (a and c) and PAM mode (b and d) in the whole-cell mode of the patch-clamp technique at a holding potential of −70 mV in CHO cells stably expressing human α4β2 (a and b) and human α7 (c and d) nAChRs. Agonist mode (a, c): 0.3% DMSO (left); 33.3 μM of the tobacco alkaloid anabasine (middle); 33.3 and 900 μM ACh for α4β2 and α7, respectively (right). PAM mode (b, d): 0.4 and 100 μM ACh for α4β2 and α7, respectively (left); co-application of 33.3 μM of the tobacco alkaloid trans-anatalline (middle); 0.4 or 100 μM ACh for α4β2 and α7, respectively (right). Thick dotted lines represent the pre-application period of the alkaloid (≥2 min). Thin dotted lines represent zero current.; PAM, positive allosteric modulator.