Fig. 1 in An electrophysiological characterization of naturally occurring tobacco alkaloids and their action on human α4β2 and α7 nicotinic acetylcholine receptors

Fig. 1. Representative traces of currents elicited in response to increasing concentrations of ACh. Currents were measured in agonist mode by using the whole-cell mode of the patch-clamp technique at a holding potential of −70 mV in CHO cells stably expressing human α4β2 (a) and human α7 (b) nAChRs. The half-maximal effective concentrations determined by using the Hill equation were 1.02 ± 1.0 μM (a) and 142 ± 16 μM (b) (n = 5–11 cells). Bars indicate (in μM): 0.1 (1), 0.4 (2), 1.2 (3), 3.7 (4), 11.1 (5), 33.3 (6), 100 (7), 300 (8), 900 (9), and 2700 (10). Dotted lines represent zero current.