Fig. 8. Siegetalis H in Diterpenoids from Sigesbeckia glabrescens with anti-inflammatory and AChE inhibitory activities

Fig. 8. Siegetalis H (11) blocks LPS-induced activation of the NF-κB pathway. (A) The phosphorylated and total protein levels of IκBα, as well as the protein level of β-actin, were quantified by immunoblotting in cell lysates of RAW264.7 cells. (B) The quantitative analysis of p- IκBα and IκBα protein levels, normalized against β-actin. (C) Immunoblot analysis of the cytoplasmic and nuclear distribution of p65 NF-κB in RAW264.7 cells. (D) Quantitative analysis of p65 protein levels, normalized against Histone H3 or β-actin. Data are presented as means ± SD, n = 3; #p <0.05, ##p <0.01, ###p <0.001, ####p <0.0001 vs control; *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001 vs LPS only.