Fig. 5 in Secotrijugins A D, four highly oxidized and rearranged limonoids from Trichilia sinensis and their anti-inflammatory activity

Fig. 5. Effects of 2 on iNOS and COX-2 expression in LPS-induced BV-2 cells. BV-2 cells were pretreated with 2 with 10, 30, and 100 μM for 30 min and then stimulated with LPS for 24 h, cells were harvested, and total protein was extracted. Protein band intensity was normalized to β-actin and was expressed as fold difference relative to the LPS group (down). ###p <0.001, compared with control, ***p <0.001; **p <0.01, *p <0.05, compared with LPS group.