Fig. 10. Compound 4 induced HepG2 cells apoptosis. HepG2 in Artemidubolides A T, cytotoxic unreported guaiane-type sesquiterpenoid dimers against three hepatoma cell lines from Artemisia dubia

Fig. 10. Compound 4 induced HepG2 cells apoptosis. HepG2 cells were treated with different concentrations (0.0, 3.5, 7.0, and 10.5 μM) of 4 for 48 h. (A) and (B) Flow cytometric analysis and cell apoptosis quantification of HepG2 cells. (C) and (D) The apoptosis-related protein levels in hepatoma cells treated with compound 4 for 48 h. Western blot and statistical results of Bax, BCL-2, PARP-1, and cleaved-PARP-1. The results were normalized to the β-actin loading control. *P <0.05, **P <0.01, and ***P <0.001, n = 3.