Fig. 9. Compound 4 in Artemidubolides A T, cytotoxic unreported guaiane-type sesquiterpenoid dimers against three hepatoma cell lines from Artemisia dubia

Fig. 9. Compound 4 induces cell cycle arrest in HepG2 cells. Cells were treated with 4 (0.0, 3.5, 7.0 and 10.5 μM) for 48 h. (A) and (B) Flow cytometric analysis and cell cycle quantification of HepG2 cells. (C) and (D) Cell cycle-associated protein levels in hepatoma cells treated with compound 4 for 48 h. Western blot and statistical results of CDK4, CDK6, CyclinD1 and P21. The results were normalized to the β-actin loading control. *P <0.05, **P <0.01, and ***P <0.001, n = 3.