Fig. 9 in Bisabolane-type sesquiterpenes from Vernonia amygdalina: Absolute configuration and anti-inflammatory activity

Fig. 9. Effects of Compounds 1–8 on the NO production and the expression of iNOS and COX-2 proteins. RAW 264.7 cells were pretreated with Compounds 1–8 for 2 h and then stimulated with 1 μg/mL LPS for additional 24 h. A: The effect of Compounds 1–8 on the viability of RAW 264.7 cells. B: The inhibitory effect of Compounds 1–8 on LPS-induced NO production in RAW 264.7 cells. C: Western blot of the expression levels of iNOS and COX-2 proteins. D–E: Quantitative densitometry analysis of iNOS/β-actin and COX-2/β-actin (mean ± SD. ###p <0.001, compared with the group untreated with LPS; *p <0.05, **p <0.01 and ***p <0.001 compared with the group treated with LPS.).