Fig. 5 in Comparative analysis of cyclotide-producing plant cell suspensions presents opportunities for cyclotide plant molecular farming

Fig. 5. Assessment of transcript or peptide levels present in O. affinis leaf, calli, and suspension cells at days 1, 14, 21 and 28. A Relative transcript expression determined by qPCR (n = 4) of asparaginyl endopeptidases OaAEP1, OaAEP2 and OaAEP3. B Relative transcript expression determined by qPCR (n = 4) of select cyclotide precursors Oak1 encoding kB1, Oak4 encoding kB2, and OaCyO2 encoding cyO2. C Relative peptide levels determined by MALDI-TOFMS (n = 4) of O. affinis cyclotides.