Fig. 6 in Furostanol saponins from Asparagus racemosus as potential hypoglycemic agents

Fig. 6. Effect of furoasparoside E (5) on Akt (Ser- 473) phosphorylation (a & b) and AMPK (Thr-172) phosphorylation (c & d) in L6 myotubes. (a and b) Cells we treated overnight with compound 5 (10 μM) with final 3 h in serum free medium and stimulated (Insulin) or not (Basal) with insulin (100 nM or 10 min) and subjected to western analysis. (c and d) Cells were treated overnight with increasing concentrations of furoasparoside E (5) and subjected to western analysis as described in methods section. Shown are representative immunoblots (a and c) and densitometric quantification of phospho-Akt relative to total Akt (b) and phospho-AMPKα relative to total AMPK (d). Results of three independent experiments are presented as mean ± S.E.M. **P <0.01 relative to control condition.