Fig. 4 in Oxylipin biosynthesis in spikemoss Selaginella moellendorffii: Identification of allene oxide synthase (CYP74L2) and hydroperoxide lyase (CYP74L1)

Fig. 4. The TIC GC–MS chromatograms of products (Me/TMS) of incubations of the recombinant CYP74L2 with 13-HPOT (A), 13-HPOD (B), 9-HPOT (C), and 9-HPOD (D). Conditions of incubation, extraction, derivatisation and analysis are described in Materials and Methods. 1a, 1b (threo and erythro isomers of 12,13-dihydroxy-9,15-octadecadienoic acid (the products of the α-ketol 1 reduction with NaBH4)); 2a, 2b (threo and erythro isomers of 12,13- dihydroxy-9-octadecenoic acid (the products of the α-ketol 2 reduction with NaBH4)); 3a, 3b (threo and erythro isomers of 9,10-dihydroxy-12,15-octadecadienoic acid (the products of the α-ketol 3 reduction with NaBH4)); 4a, 4b (threo and erythro isomers of 9,10- dihydroxy-12-octadecenoic acid (the products of the α-ketol 4 reduction with NaBH4)); 7, 9-hydroxynonanoic acid; 5, 11-hydroxy-12,13-epoxy-9-octadecenoic acid; 5a, 11-hydroxy-12,13-epoxy-9,15-octadecadienoic acid; 6, 9,10-epoxy-11-hydroxy-12-octadecenoic acid; 6a, 9,10-epoxy-11-hydroxy-12,15-octadecadienoic acid; 7, 9-hydroxynonanoic acid; 8, (9Z)-12-hydroxy-9-dodecenoic acid; 9, (10E)-12-hydroxy-10- dodecenoic acid. The structural formulae of products are present at Fig. 2. 9-HOT, (9S,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid.