Fig. 7 in Protochlorophylls in Cucurbitaceae - Distribution, biosynthesis and phylogeny

Fig. 7. (a) The unrooted phylogenetic tree of the nucleotide sequences of the chlorophyll synthase genes. The tree shows topology only. Seven different clades are distinguished. The subtree representing Clade 7 is shown with the branches occupied by two isoforms of Cucurbita pepo, C. maxima and C. moschata: C1 and C2. (b) Alignment of the chlorophyll synthase sequences of Cucurbita isoform C1, C2 and the remaining sequences of the Clade 7. The size of the letters is scaled proportionally to the numbers of the sequences that have a given amino acid in the given position. The letters are arbitrarily colour-coded to highlight similarities and differences: grey - the indicated residue is found in at least 80% of the sequences in this position in every group; green – conserved residues unique to only one group of sequences; black – variable residues, less than 80% of sequences are present in this position. The residues previously shown to play a catalytic role are highlighted in red. The position of the transmembrane helices suggested by the Uniprot database is highlighted in blue, while that predicted by the homology modelling is marked with a darkblue rectangle. (c) A 3D model of chlorophyll synthase based on the primary sequence of C. pepo subsp. pepo (NCBI: XP 023543340.1). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)