Fig. 6 in Spiroganodermaines A G from Ganoderma species and their activities against insulin resistance and renal fibrosis

Fig. 6. Compounds inhibit renal fibrosis in vitro. (A) NRK-52E cells were treated with 20 μM for 48 h, and cell viability was determined by using the CCK-8 assay. (B, E and F) Compounds at 20 μM affect renal fibrosis-related protein expression. GW788388 was used as a positive control. (C) NRK-52E cells were treated with 5, 10 and 20 μM of ()-7 for 48 h, and cell viability was determined by using the CCK-8 assay. (D, G and H) Compound ()-7 dose-dependently inhibits fibronectin and α-SMA expression. GW788388 was used as a positive control. The results were represented Mean ± SEM. *P <0.05, **P <0.01, ***P <0.001, ****P <0.0001 compared with TGF-β1 alone. #P <0.05 ##P <0.01, ###P <0.001, and ####P <0.0001 compared with DMSO alone.