Fig. 4. Compounds 1, 21 in Six C21 steroidal glycosides from Cynanchum wallichii Wight roots and their multidrug resistance reversal activities

Fig. 4. Compounds 1, 21, and 25 can reverse drug resistance by decreasing P-gp, NF-κB, and c-jun gene and protein expression. (A–C) MDR1, NFκB, and JUN gene expression in MCF-ADR and HepG2-ADM cells following co-treatment with 1, 21, and 25 and Doxorubicin. (D–E) MDR1, NFκB, and Jun protein expression in MCFADR and HepG2-ADM cells treated with 1, 21, and 25 in combination with DOX.