Fig. 5 in Spiroganodermaines A G from Ganoderma species and their activities against insulin resistance and renal fibrosis

Fig. 5. Attenuation of insulin-induced IR in myotubes. (A) C2C12 cell proliferation in response to compounds at 40 μM by CCK-8 assay. (B) C2C12 were treated with 40 μM for 24 h, and glucose uptake was determined. Metformin (Met) was used as a positive control. (C) Compound (+)-7 improves insulin-stimulated glucose uptake in IR model. (D–F) Expression of p-AKT and p-IRS1 were determined by Western blot. Compound (+)-7 improves attenuated insulin signal pathway disruption in dose-dependent manner. #P <0.05 and ###P <0.001 compared with no-insulin treatment control; *P <0.05 and **P <0.01 compared with insulin treatment alone; $$$P <0.001 and $$$$P <0.0001 compared with insulin treatment control.