Fig. 3 in Site directed mutagenesis of Catharanthus roseus (+)-vincadifformine 19-hydroxylase (CYP71BY3) results in two distinct enzymatic functions

Fig. 3. Saturation kinetics of V19H: comparative (þ)-vincadifformine saturation curve in the absence or presence of (¡)-vincadifformine. The rate of (+)-vincadifformine consumption by V19H was reduced in the presence of 3 μM of ()-vincadifformine within the range of 15–20 μM of substrate yet remains unchanged at low and saturated concentrations. Error bars indicate the standard deviation from three technical replicate assays.