Fig. 8 in Undescribed alkyne-geranylcyclohexenetriols from the endophyte Diaporthe caulivora 09F0132 and their anti-melanogenic activity

Fig. 8. Anti-melanogenic activities of caulivotrioloxin A (1) in mouse melanoma B16–F10 cells. Cells were plated with (induction) or without (basal) 50 nM α-melanocyte-stimulating hormone (α-MSH) for 24 h for inducing melanogenesis, and then incubated with the solvent control or 5, 10, 50, or 100 μM of the compound 1 for another 48 h. After treatment, cells were incubated with 10% volume of alamarBlue® reagent to determine cell viability (A), then harvested by trypsinization for analyzing melanin content (B) and tyrosinase activity (C). Arbutin (1 mM) and kojic acid (1 mM) were used as the reference control. Data were normalized with the basal group and presented as mean ± S.E.M. from at least three independent experiments. *p <0.05, **p <0.01, ***p <0.005, compared to the α-MSH-induction group.