Fig. 7 in Five undescribed steroids from Talaromyces stipitatus and their cytotoxic activities against hepatoma cell lines

Fig. 7. Apoptosis in Hep3B cells induced by compounds 4 and 8. Cells were treated with (a, e) 0.1% DMSO (control); (b, f) compound 4 at 5 μM; (c, g) compound 8 at 5 μM, and (d, h) sorafenib at 3.5 μM for a period of 24 and 48 h. The four subpopulations indicate Q4, viable; Q3, early apoptotic; Q2, late apoptotic; and Q1, necrotic. Quantification of the percentage of cells in each quadrant is shown. ***p <0.001, ****p <0.0001 compared with the control group.