Fig. 5 in Structural characterization of prenylated compounds from Broussonetia kazinoki and their antiosteoclastogenic activity

Fig. 5. Effects of 2 (A), 3 (B), and 6 (C) on RANKL-induced osteoclastogenesis in BMMs. These compounds inhibit RANKL-induced differentiation of BMMs into osteoclast. Mouse BMMs were seeded into 96 well-plates in the presence of M-CSF (30 ng/mL) and RANKL (100 ng/mL) with or without different concentrations (0, 3, 10, 30 μM) of 2, 3, and 6 for 6 days, and then cells were stained for TRAP. The quantities of TRAP-positive multinucleated (>5 nuclei) osteoclasts were determined following image capture (magnification, ×40). Data were presented as the mean ±SE (P <0.05).