Fig. 5. Forrestline F in Diterpenoid alkaloids from Delphinium forrestii var. viride and their anti-inflammation activity

Fig. 5. Forrestline F reduced LPS-induced the pro-inflammatory cytokines release and the expression of iNOS and COX-2 in RAW 264.7 cells. (A)The chemical structure of forrestline F. (B) Cell viability rate of RAW264.7 cells treated with concentration gradient of forrestline F (0–100 μM) in the presence or absence of LPS (0.5 μg/mL) for 24 h. (C) The NO inhibition curve of forrestline F, with an IC50 of 9.57 ± 1.34 μM. (D–F) Effects of forrestline F on productions of inflammatory cytokines (IL-1β, TNF-α and IL-6) in LPS-activated RAW264.7 cells. (G–I) The protein expression of iNOS and COX-2 was determined by western blotting. The values are presented as mean ± SD of three independent experiments, n = 3. ###p <0.001, vs. the control group; **p <0.01, ***p <0.001, vs. LPS-stimulated group.