Fig. 2 in Tropical vibes from Sri Lanka - cyclotides from Viola betonicifolia by transcriptome and mass spectrometry analysis

Fig. 2. Schematic representation of the general organisation of cyclotide precursors and multiple sequence alignment of 28 cyclotide precursor proteins obtained from the de novo transcriptome assembly of V. betonicifolia. A. ER signal domain at the start of each precursor sequence is highlighted in blue where cleavage of the signal domain is predicted to occur between blue and pink residues (Dutton et al., 2004). The precursor is organised with an N-terminal propeptide domain, NTPP (black), N-terminal repeat, NTR (green), mature cyclotide domain (red) and C-terminal propeptide domain, CTPP (purple). An AEP mediated cleavage potentially occurs at the conserved Asn/Asp adjacent to the CTPP of all cyclotide sequences; B. New cyclotides are named vibe 1–25. In varv A/kalata S and vibe 24 transcripts, two repeating mature domains are present. In acyclotides, either the conserved Asn/Asp or CTPP sequence is absent. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)