Fig. 5 in A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat

Fig. 5. Higher accumulation of CFG in flag leaves correlates with the presence of the Lr34res allele. A) Accumulation of CFG in Lr34 haplotypes. Twenty-two lines (Table 3) were assessed across the five haplotype groups with 5 lines tested for each of haplotypes 1–4 and 2 lines tested for haplotype 5. The median, quartiles and range of % total ion count (TIC) for the LC/MS peak corresponding to 1-O-p-Coumaroyl-3-O-feruloylglycerol (CFG) for each line (n ¼ 4) is represented, grouped by haplotype. A Kruskal-Wallis (non-parametric) analysis yielded a p-value of 0.09091. B) Accumulation of CFG in the flag leaves of five Sumai sib-pairs, grown in the field, and their two parent line 'Sumai 3' and 'Thatcher' (Table 4). Each point represents the value obtained from a different line (n ¼ 1 for each line) and an independent biological replicate, with the indicated p-value calculated by one-way ANOVA. C) Change in relative accumulations of metabolites in the Sumai Lr34þ sibs versus the Sumai Lr34- sibs, and their respective parents, grown in the fields, with Peak #'s referring to metabolites identified in Fig. 1 and Table 1. Each point represents the value obtained from a different line (n ¼ 1 for each line) and an independent biological replicate, with the significance of differences at 5% probability level determined by one-way ANOVA with LSD test post hoc. Treatments with the same letter are not significantly different.