Fig. 3 in A single residue determines substrate preference in benzylisoquinoline alkaloid N-methyltransferases

Fig. 3. Enzymatic activities of wild-type CNMT, wild-type RNMT and reciprocal 204 mutants. (A) Activity with canonical CNMT substrate (S)-coclaurine. Black bars indicate mono-methylated product (S)-N-methylcoclaurine and grey bars indicate di-methylated product (S)-N,N-dimethylcoclaurine. (B) Activity with tertiary amine substrate (S)-N-methylcoclaurine. (C) Activity with canonical RNMT substrate (S)-reticuline. Products were identified and quantified by LC-MS/MS. Error bars represent standard deviation of three replicates. WT denotes results corresponding to the wild-type enzyme.