Fig. 5 in Sesquiterpene coumarins from Ferula sinkiangensis K.M.Shen and their cytotoxic activities

Fig. 5. Apoptosis induced by Sinkiangenol E in HeLa cells detected by the Annexin V-FITC/PI staining test. HeLa cells were treated with Sinkiangenol E (0, 5, 10, 20 μM) for 24 h. DMSO treatment was used as a solvent control. (A) The apoptotic rates were determined by Annexin V-FITC/PI staining. Dot-plot graphs showed viable cells (AV-/PI-), necrotic cells (AV-/PI+), early phase apoptotic cells (AV+/PI-), and late phase apoptotic cells (AV+/PI+). Apoptosis rate was reported as the percentage of apoptotic cells (AV+/PI- and AV+/PI+) among total cells. (B) The data were presented as the mean ± SD of three independent experiments. *p <0.05 and **p <0.01 versus control were considered statistically significance.