Fig. 4 in Identification of methoxylchalcones produced in response to CuCl treatment and pathogen infection in barley

Fig. 4. Accumulation of TMC, PMC, and OPDA in barley leaves treated with 0.5 mM CuCl2 (A) and oxylipins (B) For the treatment of CuCl2, the third leaves of barley seedlings were floated in a 0.5 mM CuCl2 solution containing 0.25% Tween20, and in distilled water containing 0.25% Tween20 as a control for 72 h. For treatments with oxylipins, droplets of 1 mM solutions of JA, OPDA, and 9-KODE containing 0.25% Tween20 were placed on leaves and incubated for 72 h. Droplets of distilled water containing 0.25% Tween20 were placed on leaves as a control. Data represent mean and standard deviation values obtained from three independent samples (3 plant/sample). Asterisks indicate a statistically significant difference from the value of the control (*p <0.05; **p <0.01; Student's t-test).