Fig. 12 in Toonaones A I, limonoids with NLRP3 inflammasome inhibitory activity from Toona ciliata M. Roem

Fig. 12. In vitro bioactivity of compounds 3–14. (A) Primary screening of compounds from Toona ciliata against LDH release (n = 2). MCC950 (1 μM) was used as a positive control. Compounds with LDH release level lower than 50% were marked by arrows. (B) IC50 values of potent compounds in an LDH release assay (n = 2). These compounds were low cytotoxic with CC50 values above 20 μM. (C) Inhibition of LDH and IL-1β release (n = 2). (D) PI (propidium iodide) uptake by fluorescent microscopy (n = 1). (E–G) Western blot assays of GSDMD, Casp- 1, and IL-1β (n = 1). Compared to LPS + Nig group, *P <0.05, **P <0.01. LPS, lipopolysaccharide. Nig, Nigericin. FL, full length. NT, N-terminal.